mcd99Clones expressing mCD99 were harvested either by trypsin or using 10 mM EDTA in Hanks’ Balanced Salt Solution (HBSS). Cells were washed twice in PBS and lysated with 0.4% NP40Characterization of the interaction between mCD99 and mCD99L2. (A) Diagram of the DNA constructs for the expression of mCD99 or mCD99L2 proteins tagged with Myc, Flag,